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| Status |
Public on Jun 09, 2015 |
| Title |
Microarray analysis of HK-2 cells treated with cisplatin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, we demonstrated that cisplatin induces cell death in a proximal tubular cell line by suppressing glycolysis- and tricarboxylic acid (TCA)/mitochondria-related genes. HK-2 cells were cultured to confluence in 100mm dishes. Total RNA was extracted (QIAGEN, Valencia, CA, USA), and the concentration in the samples was measured using a Micro UV-Vis fluorescence spectrophotometer (Malcom, Tokyo, JAPAN). Sample of 10ɥg of Total RNA from HK-2 cells were labeled with biotin (3'IVT Labeling Kit, Affymetrix, USA) and hybridized (GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays, Affymetrix).
The Microarray platform used was Affymetrix HG U219 Array Strip. The arrays were scanned in a GeneAtlas scanner controlled by GeneAtlas Software (Affymetrix, Sta. Clara, USA). Genes were later filtered according to expression fold change (Affymetrix Expression Console software, Affymetrix)
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| Overall design |
Using Affymetrix GeneAtlas System, we determined the gene expression profiles of HK-2 cells treated with cisplatin. Two replicates were made for each timepoints (control, 6h and 24h).
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| Contributor(s) |
Choi S |
| Citation(s) |
26247588 |
| Submission date |
Jun 08, 2015 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Sangdun Choi |
| E-mail(s) |
sangdunchoi@ajou.ac.kr
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| Organization name |
Ajou University
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| Department |
Department of Molecular Science and Technology
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| Street address |
Woncheon-dong, Yeongtong-gu
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| City |
Suwon |
| ZIP/Postal code |
443-749 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (6)
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| GSM1705243 |
6h after cisplatin treatment, biological rep2 |
| GSM1705244 |
24h after cisplatin treatment, biological rep1 |
| GSM1705245 |
24h after cisplatin treatment, biological rep2 |
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| Relations |
| BioProject |
PRJNA286081 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69644_RAW.tar |
14.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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