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| Status |
Public on Feb 09, 2017 |
| Title |
GATA2 knockout study to investigated the role of GATA2 in human hematopoiesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We generated homozygous GATA2 knockout human embryonic stem cells (GATA2-/- hESCs) and analyzed their blood differentiation potential. Paritcularly at the hemogenic endothelium (HE) stage and hematopoietic progenitor cell (HPC) stage. Our result revealed that GATA2-/- hESCs displayed attenuated generation of CD34+CD43+ HPCs, due to the impairment of endothelial to hematopoietic transition (EHT). However, GATA2-/- hESCs retained the potential to generate erythroblasts, macrophages, but never granulocytes. Through RNA-Seq and further rescue experiment, we further identified that SPI1 was responsible for the defect of GATA2-/- hESCs in generation of CD34+CD43+ HPCs and granulocytes.
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| Overall design |
We compared the transcriptome of GATA2-/- hESCs, HE, HPC and their wildtype counterparts by RNA-Seq.
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| Contributor(s) |
Huang K, Chen Q, Shi X, Pan G, Du J |
| Citation(s) |
26246892 |
| Submission date |
Jun 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
qianyu chen |
| E-mail(s) |
chen_qianyu@gibh.ac.cn
|
| Phone |
+8602032015236
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| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
|
| Lab |
Guangjin Pan
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA286785 |
| SRA |
SRP059386 |
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