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| Status |
Public on Jan 20, 2016 |
| Title |
Transcriptome analysis of aflatoxin B1 (AFB1) induced hepatocellular carcinoma (HCC) and AFB1 resistant liver sample from rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Applied de novo assembly, both protein coding and non-coding RNAs were profiled in AFB1 induced HCC and AFB1 resistant liver sample. Compared with normal liver, the perturbation on transcriptome was revealed in multiple aspects, implying the potential mechanism of toxic resistance.
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| Overall design |
All rats were randomly divided into control and treated groups according to their weight. Then AFB1 was injected intraperitoneally to treated group in customized schedule. Biopsy was applied every 10 weeks on both groups. Tissues from rats died of HCC were reserved. All rats were sacrificed at 70th week. According to whether tumor formed, liver tissues from animals in treated group were further divided into AFB1 induced tumor sample and AFB1 resistant sample. Both samples were stored for later transcriptome analysis, as well as the normal sample from control group. RNA profiles of all 3 samples were generated by deep sequencing, using Illumina HiSeq2000 platform.
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| Contributor(s) |
Shi J, Jiang C |
| Citation(s) |
27545718 |
| Submission date |
Jun 22, 2015 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Jiejun Shi |
| E-mail(s) |
shi_ji_jun@126.com
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| Phone |
8618217441300
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| Organization name |
Tongji University
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| Street address |
No.1239, Siping Road
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA287636 |
| SRA |
SRP059729 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE70097_RAW.tar |
1.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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