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| Status |
Public on Jun 23, 2015 |
| Title |
Gene expression analysis of human bone marrow cells (hMSC-TERT) sorted from MCF7 and BT-20 co-culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have employed whole genome microarray to identify changes in gene expression in human bone marrow MSCs (hMSC-TERT) sorted from seven days co-cultures with breast cancer cell lines (MCF7) and (BT-20)
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| Overall design |
Human MSC line (hMSC-TERT) were co-cultured with breast cancer cell lines MCF7 and BT-20 for seven days then were sorted using EpiCAM MACS beads. Subsequently, RNA was extracted using Norgen total RNA isolation kit. Extracted RNA was labeled and then hybridized to the one-color Agilent Human GE 8x60K Microarray chip.
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| Contributor(s) |
Alajez NM |
| Citation(s) |
26204886 |
| Submission date |
Jun 22, 2015 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Nehad M Alajez |
| E-mail(s) |
nalajez@gmail.com
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| Organization name |
King Saud University College of Medicine
|
| Department |
Anatomy
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| Lab |
Stem Cell Unit
|
| Street address |
1st floor College of Medicne
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| City |
Riyadh |
| ZIP/Postal code |
11461 |
| Country |
Saudi Arabia |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA287651 |
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