|
| Status |
Public on Jun 23, 2015 |
| Title |
The transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide through super-enhancers to drive chondrogenesis (ChIP-Seq) |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
SOX9 is a transcriptional activator required for chondrogenesis, and SOX5 and SOX6 are closely related DNA-binding proteins that critically enhance its function. We used RNA-seq to charatierize a rat chondrosarcoma (RCS) cells as a faithful model for proliferating/early prehypertrophic growth plate chondrocytes and ChIP-seq to gain novel insights into the full spectrum of the target genes and modes of action of this chondrogenic trio.
|
| |
|
| Overall design |
ChIP-seq for SOX9, SOX6 and histone modifications were carried out using RCS cells
|
| |
|
| Contributor(s) |
Liu CF, Lefebvre V |
| Citation(s) |
26150426 |
| Submission date |
Jun 22, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Chia-Feng Liu |
| E-mail(s) |
liuc2@ccf.org
|
| Organization name |
Cleveland Clinic Lerner Research Institute
|
| Department |
Cardiovascular & Metabolic Sciences
|
| Lab |
NE3-216
|
| Street address |
9500 Euclid Avenue,
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (10)
|
|
| This SubSeries is part of SuperSeries: |
| GSE70144 |
The transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide through super-enhancers to drive chondrogenesis |
|
| Relations |
| BioProject |
PRJNA287713 |
| SRA |
SRP059755 |
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