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| Status |
Public on Jun 23, 2016 |
| Title |
The EGF Receptor Ligand Amphiregulin Controls Cell Division via FoxM1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We analyzed the effect of tetracycline (Tet)-inducible amphiregulin (AREG) silencing on the transcriptome of immortalized human N/TERT keratinocytes in the presence or absence of exogenous rhEGF. Tetracycline-mediated AREG silencing significantly altered the expression of 2,331 genes, 623 of which were not normalized towards normal by treatment with EGF. Genes irreversibly up-regulated by suppression of AREG overlapped with genes involved in keratinocyte differentiation. A significant proportion of the irreversibly down-regulated genes featured upstream binding sites recognized by FoxM1
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| Overall design |
N/TERT-2G keratinocytes with tetracycline (Tet)-inducible expression of AREG shRNA were incubated for 60h in the presence (n=3) or absence of Tet (n=3) or with Tet and 20 ng/ml recombinant EGF (n=2).
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| Contributor(s) |
Stoll SW, Elder JT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Stefan W. Stoll |
| E-mail(s) |
sstoll@umich.edu
|
| Phone |
734-763-5033
|
| Organization name |
University of Michigan
|
| Department |
Dermatology
|
| Street address |
1301 Catherine Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-5675 |
| Country |
USA |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA287924 |
| SRA |
SRP059835 |
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