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| Status |
Public on Jun 14, 2018 |
| Title |
Genome-wide chromatin localization maps of the endogenously expressed AID-labelled kinase/ATPase Rio1 in Saccharomyces cerevisiae W303, as determined under unperturbed culture conditions (YPD medium, exponential growth, 23°C) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the the first single-molecule-based NGS analysis of chromatin regions that co-immunoprecipitate with Rio1 in the yeast Saccharomyces cerevisiae.
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| Overall design |
We examined the association of Rio1-AID with chromatin by anti-AID chromatin co-immunoprecipitation followed by MiSeq deep sequencing. As the negative control experiment, we probedthe isogenic S. cerevisiae parent strain endogenously expressing untagged Rio1.
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| Contributor(s) |
Iacovella MG, Giacó L |
| Citation missing |
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| Submission date |
Jun 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter De Wulf |
| E-mail(s) |
peter.dewulf@ieo.eu
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| Phone |
+390294375036
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| Organization name |
European Institute of Oncology
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| Department |
Department of Experimental Oncology
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| Street address |
via Adamello 16
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| City |
Milan |
| ZIP/Postal code |
20139 |
| Country |
Italy |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE70287 |
The kinase/ATPase Rio1 coordinates yeast metabolism, physiology, growth and cell division at multiple levels |
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| Relations |
| BioProject |
PRJNA287950 |
| SRA |
SRP059895 |
