|
| Status |
Public on Aug 11, 2015 |
| Title |
Quantitative Genomic Analysis of RecA Protein Binding During DNA Double-Strand Break Repair Reveals RecBCD Action in vivo |
| Organism |
Escherichia coli |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here we have developed a method that combines chromatin immunoprecipitation with next-generation sequencing (ChIP-Seq) and mathematical modeling to quantify RecA protein binding during the active repair of a single DSB in the chromosome of Escherichia coli.
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| |
|
| Overall design |
Examination of RecA binding during double-strand break repair in Escherichia coli
|
| |
|
| Contributor(s) |
Cockram C, Filatenkova M, Danos V, El Karoui M, Leach D |
| Citation(s) |
26261330 |
| Submission date |
Jul 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Charlotte Anne Cockram |
| E-mail(s) |
ccockram@rockefeller.edu
|
| Organization name |
University of Edinburgh
|
| Department |
ICB
|
| Lab |
Leach Lab
|
| Street address |
Mayfield Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3JR |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL14548 |
Illumina HiSeq 2000 (Escherichia coli) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA290706 |
| SRA |
SRP061448 |
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