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| Status |
Public on Jul 30, 2007 |
| Title |
Expression data from adult laboratory mouse brain hemispheres |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inbred congenic strain B6.C6.132.54/Vad was created using C57BL/6ByJ background and BALB/cJ donor strains. Flanking background markers at chr. 6: 75.9 Mb (rs4226008, NCBI Mouse Build 36 / dbSNP Build 126) and 122.3 Mb (rs3023093), and limiting donor markers at 81.9 Mb (rs4226024) and at 91.8 Mb (rs3712161) defined the introgressed region. We concluded the segment size must be between 9.9 Mb and 46.4 Mb. In a Quantitative Trait Gene identification study we compared brain (without cerebellum) gene expression between progenitors and congenics. Such comparisons can facilitate identification of cis-regulated genes and to establish genetic control of a complex phenotype whose expression is associated with the introgressed chromosome segment.
We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process.
Keywords: strain comparison
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| Overall design |
Experiments were carried out in three batches. In each batch 10 animals per strain were used. After processing each hemisphere separately, 10 hemispheres were pooled for one high-density oligonucleotide microarray (Mouse Genome 430 2.0, Affymetrix, Santa Clara, CA). For each strain 3 oligonucleotide microarrays were used (n=3). We used adult, untreated, male mice (n=30, 90 mice in toto).
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| Contributor(s) |
Vadasz C, Saito M |
| Citation(s) |
17936574 |
| Submission date |
Feb 28, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Csaba Vadasz |
| E-mail(s) |
vadasz@nki.rfmh.org
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| Organization name |
New York University NKI
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| Department |
Center for Neurobehavior Genetics
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| Street address |
140 Old Orangeburg Road
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| City |
Orangeburg |
| State/province |
NY |
| ZIP/Postal code |
10962 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| GSM172395 |
C57BL/6ByJ strain, brain hemisphere, biological rep1 |
| GSM172396 |
C57BL/6ByJ strain, brain hemisphere, biological rep2 |
| GSM172397 |
C57BL/6ByJ strain, brain hemisphere, biological rep3 |
| GSM172398 |
BALB/cJ strain, brain hemisphere, biological rep1 |
| GSM172399 |
BALB/cJ strain, brain hemisphere, biological rep2 |
| GSM172400 |
BALB/cJ strain, brain hemisphere, biological rep3 |
| GSM172401 |
B6By.C.132.54 strain, brain hemisphere, biological rep1 |
| GSM172402 |
B6By.C.132.54 strain, brain hemisphere, biological rep2 |
| GSM172403 |
B6By.C.132.54 strain, brain hemisphere, biological rep3 |
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| Relations |
| BioProject |
PRJNA98273 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7155_RAW.tar |
28.3 Mb |
(http)(custom) |
TAR (of CEL) |
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