|
| Status |
Public on Aug 04, 2015 |
| Title |
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain [mouse NSC miRNA 2.0] |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
We performed a global analysis of extracellular miRNAs in the serum of human subjects diagnosed with Alcohol Use Disorder (AUD) to identify robust biomarkers of early brain damage or dysfunction. This was performed in a set of 20 AUD subjects and 10 age-matched controls. They were subjected to comprehensive medical, neuropsychological and neuroimaging tests, followed by comparison of miRNA levels found in peripheral blood serum. The levels of miRNAs were quantified using two independent high-throughput methods: Affymetrix microarray and Illumina next-generation RNA-sequencing. Cross-species validation was performed using rat drinking models and mouse neural stem cell culture models, with tissue specificity of the serum miRNAs examined using additional RNA-sequencing of serum and body tissues in untreated rats. This data series includes the mouse neural stem cell miRNA 2.0 GeneChip data only but is part of a larger SuperSeries (GSE71579).
|
| |
|
| Overall design |
A total of 14 miRNA 2.0 GeneChips were run on small RNA purified from the mouse neural stem cell samples.
|
| |
|
| Contributor(s) |
Middleton FA, Ignacio C, Hicks SD, Burke P, Lewis L, Szombathyne-Meszaros Z |
| Citation(s) |
26341662 |
| Submission date |
Aug 03, 2015 |
| Last update date |
May 02, 2017 |
| Contact name |
Frank A Middleton |
| E-mail(s) |
middletf@upstate.edu
|
| Phone |
315-464-7721
|
| Organization name |
SUNY Upstate Medical Univ
|
| Department |
Neuroscience and Physiology
|
| Street address |
505 Irving Avenue
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
|
| Samples (18)
|
| GSM1842415 |
Mouse NSC treated with FGF+ethanol replicate 1 |
| GSM1842416 |
Mouse NSC treated with FGF+ethanol replicate 2 |
| GSM1842417 |
Mouse NSC treated with FGF+ethanol replicate 3 |
| GSM1842418 |
Mouse NSC treated with FGF+ethanol replicate 4 |
| GSM1842419 |
Mouse NSC treated with FGF replicate 1 |
| GSM1842420 |
Mouse NSC treated with FGF replicate 2 |
| GSM1842421 |
Mouse NSC treated with FGF replicate 3 |
| GSM1842422 |
Mouse NSC treated with FGF replicate 4 |
| GSM1842423 |
Mouse NSC treated with TGF+ethanol replicate 1 |
| GSM1842424 |
Mouse NSC treated with TGF+ethanol replicate 2 |
| GSM1842425 |
Mouse NSC treated with TGF+ethanol replicate 3 |
| GSM1842426 |
Mouse NSC treated with TGF+ethanol replicate 4 |
| GSM1842427 |
Mouse NSC treated with TGF+ethanol replicate 5 |
| GSM1842428 |
Mouse NSC treated with TGF replicate 1 |
| GSM1842429 |
Mouse NSC treated with TGF replicate 2 |
| GSM1842430 |
Mouse NSC treated with TGF replicate 3 |
| GSM1842431 |
Mouse NSC treated with TGF replicate 4 |
| GSM1842432 |
Mouse NSC treated with TGF replicate 5 |
|
| This SubSeries is part of SuperSeries: |
| GSE71579 |
Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain |
|
| Relations |
| BioProject |
PRJNA291744 |
Less...
More...