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| Status |
Public on Mar 21, 2007 |
| Title |
Structural basis for recognition of SMRT/N-CoR by the MYND domain and its contribution to AML1/ETO's activity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To examine the effects of disrupting the AML1/ETO MYND-SMRT interaction with the W692A substitution on AML1/ETO function, the global gene expression profile of mouse bone marrow LSK cells transduced with GFP was compared to that of cells transduced with either wild-type AML1/ETO or AML1/ETO harboring the W692A substitution in the MYND domain. Three independent biological replicates were assessed for both the control (GFP/MigR1) and AML1/ETO (intact MYND-SMRT interaction) conditions, whereas four independent biological replicates were assessed for the W692A (disrupted MYND-SMRT interaction) condition. The three GFP replicates were used to establish a baseline signal for comparison to both the AML1/ETO and W692A samples.
Keywords: genetic modification
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| Overall design |
Global gene expression profiles of FACS-sorted Lin-Sca1+cKit+ mouse bone marrow cells transduced with empty vector (GFP-MigR1), AML1/ETO, or AML1/ETO with the W692A substitution (W692A).
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| Contributor(s) |
Speck NA, J Gaudet J, Liu Y, Chen W, Cheney MD, Roudaia L, Cierpicki T, Klet RC, Hartman K, Laue TM, Bushweller JH |
| Citation(s) |
17560331 |
| Submission date |
Mar 20, 2007 |
| Last update date |
Aug 29, 2019 |
| Contact name |
Nancy Asmussen Speck |
| E-mail(s) |
Nancy.Speck@dartmouth.edu
|
| Phone |
(603) 650-1235
|
| Fax |
(603) 650-1129
|
| Organization name |
Dartmouth Medical School
|
| Department |
Biochemistry
|
| Lab |
Speck Lab
|
| Street address |
311 Vail Bldg
|
| City |
Hanover |
| State/province |
NH |
| ZIP/Postal code |
03755 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA98039 |
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