|
| Status |
Public on Apr 11, 2016 |
| Title |
82-kDa ChAT and SATB1 ChIP-seq after vehicle or amyloid-beta exposure |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Using chromatin immunoprecipitation and next-generation sequencing (ChIP-seq), we assessed the effects of acute exposure to oligomeric amyloid-beta on 82-kDa ChAT and SATB1 genome association in human SH-SY5Y neural cells, finding that Aβ-exposure increased 82-kDa ChAT and SATB1 association with gene promoters, introns and matrix attachment regions. We found that both SATB1 and 82-kDa ChAT associate with synapse and cell stress related genes after amyloid-beta exposure.
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| |
|
| Overall design |
Examination of effects of amyloid-beta exposure on 82-kDa ChAT and SATB1 in human SH-SY5Y neural cells
|
| |
|
| Contributor(s) |
Rylett RJ, Winick-Ng W |
| Citation(s) |
27052102 |
| Submission date |
Sep 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sandeep K Gill |
| Organization name |
Robarts Research Institute
|
| Department |
Molecular Medicine
|
| Street address |
100 Perth Drive
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A5K8 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (6)
|
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| Relations |
| BioProject |
PRJNA297352 |
| SRA |
SRP064320 |
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