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| Status |
Public on Jan 31, 2016 |
| Title |
Expression profiling individual DrosDel flies heterozygous for deletions of chromosome 2L in a hybrid background |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to understand the effect of genetic background on the response to gene dose perturbation, we performed mRNA transcriptional profiling on 99 hemizygotic lines (Df/+) from the DrosDel project, which have hybrid genetic background of OregonR/w1118.
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| Overall design |
We performed RNA-Seq analysis of 417 single adult flies in duplicate or triplicate. Flies are from 73 different genotypes. Differential gene expression was analyzed separately for each sex, gene expression from each genotype was compared to normalized mean of gene expression remaining 72 genotypes.
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| Contributor(s) |
Lee H, Whitworth C, Oliver B |
| Citation(s) |
27599372, 29242386 |
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Hangnoh Lee, Dong-Yeon Cho, Cale Whitworth, Robert Eisman, Melissa Phelps, John Roote, Thomas Kaufman, Kevin Cook, Steven Russell, Teresa M Przytycka, Brian Oliver. Genome-wide expression profiling Drosophila melanogaster deficiency heterozygotes reveals diverse genomic responses. bioRxiv doi: http://dx.doi.org/10.1101/032920
Dong-Yeon Cho, Hangnoh Lee, Damian Wojtowicz, Steven Russell, Brian Oliver, Teresa M Przytycka. Interplay between copy number, dosage compensation and expression noise in Drosophila. bioRxiv doi: https://doi.org/10.1101/041038
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| Submission date |
Oct 12, 2015 |
| Last update date |
Aug 13, 2019 |
| Contact name |
Brian Oliver |
| E-mail(s) |
briano@nih.gov
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| Phone |
301-204-9463
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| Organization name |
NIDDK, NIH
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| Department |
LBG
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| Lab |
Developmental Genomics
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| Street address |
50 South Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (417)
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| Relations |
| BioProject |
PRJNA298537 |
| SRA |
SRP064744 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE73920_RAW.tar |
220.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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