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| Status |
Public on Aug 01, 2008 |
| Title |
TfRc Dependent Gene Expression in Embryonic Renal Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We investigated the ability of transferrin receptor1 (TfRc) knockout cells to populate different domains of the developing kidney by using a chimeric approach. The TfRc cells developed into all segments of the developing nephron, but there was a relative exclusion from the ureteric bud and a positive bias towards the stromal compartment. Here we conducted a microarray analysis of differential gene expression between TfRc deficient and wild type (wt) cells in chimeric embryonic kidneys derived from embryos created by blastocyst injection of wt blastocysts with TfRc-/- green fluorescent protein-expressing (GFP+) embryonic stem cells.
Keywords: cell type comparison, genetic modification, iron signaling
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| Overall design |
Following blastocyst injection of 3-5 TfRc-/- GFP+ embryonic stem cells in wt blstocysts, we harvested the chimeric kidney at day E15.5 and separated the TfRc-/- GFP+ cell population from wt GFP- cell population by using fluorescence activated cell sorting. Biotinlyated cRNA was prepared from the isolated cells and hybridized to Mouse Genome 430 2.0 Arrays (Affymetrix) according to standard protocols.
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| Contributor(s) |
Li J, Paragas N, Schmidt-Ott KM, Chen X, Barasch J |
| Citation(s) |
19154717 |
| Submission date |
Mar 29, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jonathan Barasch |
| E-mail(s) |
jmb4@columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
630 West 168th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
|
| GSM177573 |
wildtype (GFP-negative) kidney cells, biological rep1 |
| GSM177574 |
wildtype (GFP-negative) kidney cells, biological rep2 |
| GSM177575 |
Tfrc-/- (GFP-positive) kidney cells, biological rep1 |
| GSM177576 |
Tfrc-/- (GFP-positive) kidney cells, biological rep2 |
|
| Relations |
| BioProject |
PRJNA97885 |
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