|
| Status |
Public on Apr 20, 2016 |
| Title |
Gene expression from splenic cells in response to VSV-M2 and ligands to RIG-I and MDA5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
VSV-M2 is recognized by cytosolic RIG-I. Notably, 5'-triphosphate RNA molecules derived from either viral RNA or from the synthetically produced 3pRNA can also induce RIG-I activation. MDA5 stimulation is achieved using complexed poly(I:C), a synthetic analog of viral dsRNA.
To test whether the RIG-I and MDA5 ligands 3pRNA and poly(I:C) can be used in their complexed structures to decipher RNA virus-induced sickness behavior in vivo, we first compared the tissue-specific signaling pathways after systemic challenge with VSV-M2 and the RIG-I and MDA5 ligands, respectively. A whole-genome expression analysis using splenic cells was carried out using an Affymetrix Mouse Gene 2.1 ST Array.
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| Overall design |
Adult wt mice were injected with either VSV-M2, 3pRNA or complexed poly(I:C); 18h (VSV) or 24h later spleens were removed for RNA extraction and hybridization on Affymetrix arrays.
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| Contributor(s) |
Spiess A, Hagemeyer N, Blank T, Staszewski O, Detje C |
| Citation(s) |
27096319 |
| Submission date |
Oct 14, 2015 |
| Last update date |
Mar 08, 2018 |
| Contact name |
Ori Staszewski |
| E-mail(s) |
ori.staszewski@uniklinik-freiburg.de
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Institute of Neuropathology
|
| Street address |
Breisacher Str. 64
|
| City |
Freiburg |
| ZIP/Postal code |
D-79106 |
| Country |
Germany |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
|
| Samples (19)
|
| GSM1908186 |
Splenocytes_18h_post_VSVinPBS (A912b_02) |
| GSM1908187 |
Splenocytes_18h_post_VSVinPBS (A912b_04) |
| GSM1908188 |
Splenocytes_18h_post_PBS (A912b_06) |
| GSM1908189 |
Splenocytes_18h_post_PBS (A912b_07) |
| GSM1908190 |
Splenocytes_18h_post_PBS (A912b_08) |
| GSM1908191 |
Splenocytes_24h_post_poly(I:C)inJetPEI (A912_09) |
| GSM1908192 |
Splenocytes_24h_post_poly(I:C)inJetPEI (A912_10) |
| GSM1908193 |
Splenocytes_24h_post_poly(I:C)inJetPEI (A912_11) |
| GSM1908194 |
Splenocytes_24h_post_3pRNAinJetPEI (A912_12) |
| GSM1908195 |
Splenocytes_24h_post_3pRNAinJetPEI (A912_13) |
| GSM1908196 |
Splenocytes_24h_post_3pRNAinJetPEI (A912_14) |
| GSM1908197 |
Splenocytes_24h_post_JetPEI (A912_15) |
| GSM1908198 |
Splenocytes_24h_post_JetPEI (A912_16) |
| GSM1908199 |
Splenocytes_24h_post_JetPEI (A912_17) |
| GSM1908200 |
Splenocytes_18h_post_PBS (A912_18) |
| GSM1908201 |
Splenocytes_18h_post_PBS (A912_20) |
| GSM1908202 |
Splenocytes_18h_post_VSVinPBS (A912_21) |
| GSM1908203 |
Splenocytes_18h_post_VSVinPBS (A912_22) |
| GSM1908204 |
Splenocytes_18h_post_VSVinPBS (A912b_01) |
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| This SubSeries is part of SuperSeries: |
| GSE74063 |
Brain Endothelial Specific IFNAR Is Key For Virus-Induced Sickness Behavior And Cognitive Impairment |
|
| Relations |
| BioProject |
PRJNA298744 |
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