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| Status |
Public on May 26, 2016 |
| Title |
Transcription Profiles of Regulatory T Cells in Follicular Lymphoma Lymph Nodes, Normal Lymph Nodes and Reactive Lymph Nodes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have previously shown that Tregs infiltrating follicular lymphoma lymph nodes (FLN) are quantitatively and qualitatively different than those infiltrating normal and reactive nodes (NLN, RLN, respectively). To gain insight into how such Treg populations differ, we performed RNA sequence (RNAseq) analyses on flow sorted Tregs from all three sources. We identify several molecules that could contribute to the observed increased suppressive capacity of FLN tregs, including upregulation of CTLA-4, IL-10, and GITR, all confirmed by protein expression. In addition, we identify, and confirm functionally, a novel mechanism by which Tregs target to and accumulate within a human tumor microenvironment, through the down regulation of S1PR1, SELL (L-selectin) and CCR7, potentially resulting in greater lymph node retention. In addition we identify and confirm functionally the upregulation of CXCR5, CXCL13 and IL-16 demonstrating the unique ability of the follicular derived Tregs to localize and accumulate within not only the malignant lymph node, but also localize and accumulate within the malignant B cell follicle itself. Such findings offer significant new insights into how FLN Tregs may contribute to the biology of follicular lymphoma and identify several novel therapeutic targets.
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| Overall design |
Cross sectional comparison of transcription profiles of human regulatory T cells from normal lymph nodes (n=10), follicular lymphoma lymph nodes (n=12) and reactive lymph nodes (n=5).
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| Contributor(s) |
Nedelkovska H, Rosenberg AF, Hilchey SP, Hyrien O, Burack WR, Quataert SA, Baker CM, Azadniv M, Welle SL, Ansell SM, Kim M, Bernstein SH |
| Citation(s) |
27228053 |
| Submission date |
Oct 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Alexander Rosenberg |
| E-mail(s) |
afr@uab.edu
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| Organization name |
University of Alabama at Birmingham
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| Street address |
1720 2nd Avenue South, Suite 276/11
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| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
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| Platforms (1) |
| GPL13393 |
AB SOLiD 4 System (Homo sapiens) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA299009 |
| SRA |
SRP064912 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74102_Treg_FL_gene_expression_data_matrix.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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