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| Status |
Public on Apr 18, 2016 |
| Title |
Gene expression profiling of D. melanogaster strains with varied resistance and tolerance to L. monocytogenes infection |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We sequenced the mRNA of four D. melanogaster strains (w1118:BL6326, PBacCG2247:BL18050, PBacPCMT:BL18398, and RAL359:BL28131) during L. monocytogenes infection to determine the changes in gene expression dynamics responsible for resistance and tolerance phenotypes.
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| Overall design |
Examination of mRNA levels in groups of 20 adult males at 12 timepoints throughout infection and recovery. Flies were injected with 1000CFU of L. monocytogenes. On day 3 and onwards, flies were maintained on ampicillin supplemented food.
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| Contributor(s) |
Louie A, Schneider D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Alexander Louie |
| Organization name |
Stanford University
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| Department |
Microbiology and Immunology
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| Lab |
Schneider
|
| Street address |
299 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (63)
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| Relations |
| BioProject |
PRJNA299012 |
| SRA |
SRP064917 |
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