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| Status |
Public on Apr 04, 2007 |
| Title |
Investigate the role of Wnt/beta-catenin signaling in development of the exocrine pancreas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
beta-catenin is an essential mediator of canonical Wnt signaling and a central component of the cadherin-catenin epithelial adhesion complex. Dysregulation of beta-catenin expression has been described in pancreatic neoplasia. Newly published studies have suggested that beta-catenin is critical for normal pancreatic development although these reports reached somewhat different conclusions. In addition, the molecular mechanisms by which loss of beta-catenin affects pancreas development are not well understood. The goals of this study then were; 1] to further investigate the role of beta-catenin in pancreatic development using a conditional knockout approach and 2] to identify possible mechanisms by which loss of beta-catenin disrupts pancreatic development. A Pdx1-cre mouse line was used to delete a floxed beta-catenin allele specifically in the developing pancreas, and embryonic pancreata were studied by immunohistochemistry and microarray analysis.
Keywords: Time course
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| Overall design |
Parameter: embryonic pancreas from wild type and Pdx1Cre/+; beta-cateninflox/flox
Sample type: RNA
Source name: Embryonic day 14.6 and 16.5 pancreas
Organism: Mus Musculus
Strain: Derived from an intercross of Pdx1Cre/+ ; beta-cateninflox/+ mice.
Extracted molecule: Total RNA
In the study, we hybridized RNA from Embryonic day 14.6 and 16.5 pancreas of wild type (WT) control and Pdx1Cre/+; beta-cateninflox/flox beta-catenin null in the pancreas) embryonic pancreas to Affymetrix MOE430 2.0 GeneChip® arrays containing 45,101 well characterized mouse genes/ESTs.
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| Contributor(s) |
Wells JM, Aronow BJ |
| Citation(s) |
17222338 |
| Submission date |
Apr 02, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Bruce J Aronow |
| E-mail(s) |
bruce.aronow@chmcc.org
|
| Phone |
513-636-4865
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA97951 |
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