Expression profiling by high throughput sequencing
Summary
RNA-Seq was used to assess the gene expression profiles of 4 allodiploid embryonic stem cell lines and 4 control embryonic stem cell lines. Moreover, single-cell RNA-Seq was used to quantify the transcriptomes of 87 allodiploid single cells.
Overall design
Comparison of mRNA profiles of 4 allodiploid embryonic stem cell lines to 4 control mouse and rat embryonic stem cell lines using the Illumina HiSeq 2000 platform. Single-cell RNA-Seq was conducted to check the transcriptomes of single allodiploid embryonic and differentiated cells.
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