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| Status |
Public on Dec 04, 2015 |
| Title |
ChIP-Seq for Yrr1 protein on Saccharomyces cerevisiae cells carrying different YRR1 alleles in response to 4-nitroquinoline-N-oxide (4NQO) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In this study, we constructed three isogenic strains of S96 yrr1Δ background (its native YRR1 gene was knocked out) carrying three different YRR1 alleles, YRR1_S96, YRR1_YJM789, YRR1_S96-I775E, respectively. We then conducted chromatin immuno-precipitation followed by high-throughput sequencing (ChIP-Seq) for Yrr1 protein on the three strains grown in Yeast Peptone Dextrose medium (YPD) and YPD + 4NQO.
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| Overall design |
ChIP-Seq for Yrr1 protein was performed in biological triplicates on S96 cells carrying three different YRR1 alleles grown in YPD and YPD + 4NQO. Immune-precipitated DNA were sequenced for all the three cultures and input genomic DNA was sequenced for one culture.
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| Contributor(s) |
Gallagher JE, Rong-Mullins X, Dunn BL, Jiang L, Snyder MP |
| Citation(s) |
24532717, 29208650 |
| Submission date |
Nov 05, 2015 |
| Last update date |
Feb 13, 2020 |
| Contact name |
Xiaoqing Rong-Mullins |
| Organization name |
West Virginia University
|
| Street address |
53 Campus Dr
|
| City |
Morgantown |
| State/province |
West Virginia |
| ZIP/Postal code |
26505 |
| Country |
USA |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA301204 |
| SRA |
SRP065829 |
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