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| Status |
Public on Nov 11, 2015 |
| Title |
Genome-wide analysis of HoxBlinc lncRNA downstream targets in mouse ESC-derived embryonic bodies |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We find a new non-coding transcript associated with active HoxB locus, which we named HoxBlinc lncRNA. We report that HoxBlinc RNA specifies Flk1+ mesoderm and then promotes the development of HS/PCs and cardiomyocytes. To understand the molecular pathways reguated by HoxBlinc, we generate two doxycycline inducible KD ES R1E cell lines, then differentiate these cell lines to day 6 via embryonic bodies stage. RNA-seq analysis were performed for day 6 EBs with or without doxycycline treatment starting at Flk1+ mesodermal stage.
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| Overall design |
RNA from day 6 EBs targeting with 2 invidiual shRNAs with or without doxycycline treatment were combined together for RNA-seq analysis.
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| Contributor(s) |
Deng C, Li Y, Zhou L, Cho J, Patel B, Terada N, Li Y, Bungert J, Qiu Y, Huang S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Lei Zhou |
| E-mail(s) |
leizhou@ufl.edu
|
| Phone |
3522738169
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| Organization name |
University of Florida
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Zhou
|
| Street address |
PO Box 100266
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA301737 |
| SRA |
SRP066023 |
