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| Status |
Public on Jan 10, 2016 |
| Title |
Examination of SUUR and Pc chromatin proteins in slivary glands in presence or absence of SuUR mutation using DamID technique |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Heterochromatin contains repressively modified histones and replicates late in S phase of the cell cycle. Besides the shortage in replication origins, little is known about replication timing regulation in silenced regions. In Drosophila polytene cells, late replication results in under-replication and decreased DNA copy number in heterochromatic regions of the genome. The Suppressor of Under-replication (SUUR) protein controls this feature – in its absence the DNA polytenization level in most silenced regions is restored, however the repressive histone marks are lost. We hypothesized that SUUR regulates the re-establishment of repressive histone pattern during replication which results in delayed replication completion of heterochromatin. Measuring DNA copy number in mutants with disrupted repressive pathways, we found that under-replication is directly linked to repressive histone marks supply. DamID-seq and ChIP-seq experiments revealed that SuUR mutation does not affect the establishment of heterochromatin domains. Here, we identified a novel SUUR protein interaction (CG12018) that supports SUUR association with replication complex. SUUR loads onto replication forks shortly after the origin firing and participates in chromatin maintenance rather than its establishment. Thus, our findings provide comprehensive evidence that late replication in Drosophila is caused by the time-consuming process of replication-coupled repressive chromatin renewal.
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| Overall design |
Examination of SUUR and Pc chromatin proteins in slivary glands in presence or absence of SuUR mutation using DamID technique
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| Contributor(s) |
Posukh OV, Belyakin SN, Maksimov DA, Koryakov DE, Skvortsova KN, Pokholkova GV |
| Citation(s) |
29191233 |
| Submission date |
Nov 11, 2015 |
| Last update date |
Jul 19, 2019 |
| Contact name |
Daniil Maksimov |
| E-mail(s) |
vift@mail.ru
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| Organization name |
MCB NSC RAS
|
| Lab |
Genomics lab
|
| Street address |
Lavrentjeva ave., 8/2
|
| City |
Novosibirsk |
| ZIP/Postal code |
630090 |
| Country |
Russia |
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| Platforms (2) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE74909 |
Repressive Histone Marks Renewal Impedes Replication Fork Progression and Contributes To Late Replication in Drosophila |
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| Relations |
| BioProject |
PRJNA301887 |
| SRA |
SRP066049 |
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