|
| Status |
Public on Jun 01, 2016 |
| Title |
Inhibition of uPA expression by CRISPR-dCas9 DNA methyltransferases |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the generation of CRISPR-dCas9 DNA methyltransferases to mediate targeted DNA methylation. Using the dCas9-BFP-DNMT3A and dCas9-BFP-DNMT3B methyltransferases, we have demonstrated that these two methyltransferase can mediate targeted methylation in three human genes tested: uPA, TGFBR3, and CDKN2A in human HEK293T cells. We also showed that these methyltransferases could mediate gene inhibition.
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| |
|
| Overall design |
five samples co-transfected with five uPA sgRNAs and each of the four dCas9 fusions, or control transfection with pUC19 plasmid
|
| |
|
| Contributor(s) |
Luo Y |
| Citation(s) |
29635374 |
| Submission date |
Nov 12, 2015 |
| Last update date |
Dec 25, 2019 |
| Contact name |
Yonglun Luo |
| E-mail(s) |
alun@biomed.au.dk
|
| Phone |
0045-22411944
|
| Organization name |
Aarhus University
|
| Street address |
Wilhelm Meyers Allé 4
|
| City |
aarhus |
| ZIP/Postal code |
8000 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA301943 |
| SRA |
SRP066095 |
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