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| Status |
Public on Aug 01, 2007 |
| Title |
Effects of JNK on TGF-beta responses of mouse B lymphoma derived A20 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
TGF-beta plays multiple functions in a board range of cellular responses such as proliferation, differentiation, motility and survival by activating several cellular signaling pathways, including Smads and MAP kinases (Erk, JNK and p38). In particular, TGF-beta can activate pro- or anti-apoptotic signals depending on the target cells. We found that blockage of JNK activation sensitized mouse B lymphoma derived A20 cells to TGF-beta-induced apoptosis. These results suggest that TGF-beta activate JNK to inhibit the activation of death signal that is simultaneously activated by TGF-beta.
We used microarrays to gain insight into the effects of JNK inhibition on gene expression in TGF-b-stimulated A20 cells and identified JNK-dependent TGF-beta inducible genes.
Keywords: time course
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| Overall design |
The following six samples were prepared: untreated A20 cells (non-stimulated, DMSO): A20 cells cultured with SP600125 for 24 h (non-stimulated, SP600125): A20 cells stimulated with TGF-beta for 12 h (TGF-beta 12 h, DMSO) and 24 h (TGF-beta 24 h, DMSO): and A20 cells stimulated with TGF-beta in the presence of SP600125 for 12 h (TGF-beta 12 h, SP600125) and 24 h (TGF-beta 24 h, SP600125), respectively. Total RNA was prepared and hybridized to the Affymetrix Mouse Genome 430 2.0 array. Genes whose expression was increased by more than 2-fold at either 12 or 24 h after TGF-beta stimulation were identified as TGF-beta inducible genes. Amongst them, we identified genes whose induction levels were reduced by more than 75% by co-treatment with the JNK inhibitor SP600125.
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| Contributor(s) |
Ohgushi M, Yamamoto T, Nishida E, Yonehara S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 12, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
shin yonehara |
| E-mail(s) |
yonehara@lif.kyoto-u.ac.jp
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| Organization name |
Graduate School of Biostudies, Kyoto University
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| Street address |
Yoshida Konoe-cho, Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA100489 |
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