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| Status |
Public on Nov 24, 2017 |
| Title |
Zac1 is a regulator of the imprinted gene network (ChIP-seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In order to determine the imprinted transcription factor Zac1 targets, we overexpressed Zac1 in a neuroblastoma cell line and measured both the regulated expressed genes by Differential Gene Expressed analysis and Zac1 binding sites throughout the mouse genome by ChIP-seq. We have shown that Zac1 regulates and binds closed to many genes belonging to the Imprinted Gene Network.
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| |
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| Overall design |
Determination of Zac1 binding sites in transfected Neuro-2A cells (2 biolobical replicates) using ChIP-seq, with the chromatin input as a control.
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| |
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| Contributor(s) |
Varrault A, Dantec C, LeDigarcher A, Chottard L, Parrinello H, Dubois E, Bouschet T, Journot L |
| Citation(s) |
28985358 |
| Submission date |
Dec 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Tristan Bouschet |
| E-mail(s) |
tristan.bouschet@igf.cnrs.fr
|
| Phone |
+33(0)4 3435 9240
|
| Organization name |
Institut de Génomique Fonctionnelle
|
| Department |
CNRS 5203, INSERM U661
|
| Lab |
Functional Genomics of Imprinted Genes
|
| Street address |
141 rue de la cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34094 |
| Country |
France |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (4)
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| GSM1970492 |
Immunoprecipitated chromatin replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE76032 |
Genome-wide characterization of Zac1 target genes reveals its role as a broad regulator of the imprinted gene network including extracellular matrix genes |
|
| Relations |
| BioProject |
PRJNA305755 |
| SRA |
SRP067280 |
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