|
| Status |
Public on Jun 12, 2017 |
| Title |
The role of Sgf73 occupancy |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Sgf73, a core component of the SAGA (Spt-Ada-Gcn5 Acetyltransferase) co-activator complex, is the yeast orthologue of ataxin-7, which in humans undergoes CAG ? polyglutamine repeat expansion to produce the neurodegenerative disease spinocerebellar ataxia type 7 (SCA7). We recently documented that deletion of SGF73 dramatically extends replicative lifespan (RLS) in yeast. To define the basis for Sgf73-mediated RLS extension, and to further explore the molecular function of Sgf73/ataxin-7 we performed ChIP-Seq for Sgf73 in yeast to identify its regions of DNA binding. The aim of the study was to uncover the underlying mechanisms controlled by Sgf73. This information will be used to understand the role of Sgf73 target genes in yeast aging pathways and possibly highlight pathways of mammalian aging regulation and SCA7 neurodegeneration in humans.
|
| |
|
| Overall design |
Examination of Sgf73 occupancy in wildtype S. cerevisiae
|
| Web link |
http://onlinelibrary.wiley.com/doi/10.1111/acel.12611/abstract;jsessionid=72C8B7B277DB98289BB038D59120160B.f02t02
|
| |
|
| Contributor(s) |
Mason AG, Garza RM, McCormick MA, Kennedy BK, Pillus L, La Spada AR |
| Citation(s) |
28568901 |
| Submission date |
Dec 31, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Albert R. La Spada |
| Organization name |
University of California San Diego
|
| Department |
Pediatrics
|
| Street address |
2880 Torrey Pines Scenic Dr.
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA307564 |
| SRA |
SRP068020 |
Less...
More...