|
| Status |
Public on Jan 13, 2017 |
| Title |
Vitamin D/Vitamin D Receptor (VDR) Axis Regulates LMNA gene expression [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP with VDR antibody followed by DNA sequencing to determine if VDR binds the LMNA gene locus
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| |
|
| Overall design |
BJ fibroblasts were treated with 10-7 M 1,25D or FBS (vehicle) for 24 h, and cells crosslinked, sonicated, and chromatin isolated to perform ChIP analyses as described in Gonzalez-Suarez et al. EMBO Journal 2009. Chromatin was immunoprecipitated using anti-VDR antibody (10 mg) or IgG (10 mg) as control.
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| |
|
| Contributor(s) |
Dorsett D, Kreienkamp R, Gonzalo S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Susana Gonzalo |
| E-mail(s) |
sgonzalo@slu.edu
|
| Phone |
314-997-9244
|
| Organization name |
Saint Louis University
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
1100 S Grand Blvd
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
|
| Samples (4)
|
| GSM2039945 |
vitamin D receptor ChIP vitamin D treated |
|
| This SubSeries is part of SuperSeries: |
| GSE76887 |
Vitamin D/Vitamin D Receptor (VDR) Axis Regulates LMNA gene expression |
|
| Relations |
| BioProject |
PRJNA308854 |
| SRA |
SRP068474 |
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