|
| Status |
Public on Feb 09, 2017 |
| Title |
MicroRNAs circulate in the hemolymph of Drosophila and accumulate relative to tissue microRNAs in an age-dependent manner [mRNA] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In mammals, extracellular miRNAs circulate in biofluids as stable entities that are secreted by normal and diseased tissues, and can enter cells and regulate gene expression. Drosophila melanogaster is a proven system for the study human diseases. They have an open circulatory system in which hemolymph (HL) circulates in direct contact with all internal organs, in a manner analogous to vertebrate blood plasma. Here we show using deep sequencing that Drosophila HL contains RNase resistant, circulating miRNAs (HL-miRNAs). Limited subsets of body tissue miRNAs (BT-miRNAs) accumulated in HL, suggesting they may be specifically released from cells or particularly stable in HL. Alternatively, they might arise from specific cells such as hemocytes, in intimate contact with HL. Young and old flies accumulated unique populations HL-miRNAs, suggesting their accumulation is responsive to the physiological status of the fly. These HL-miRNAs may function in flies similarly to the miRNAs circulating in mammalian biofluids. The discovery of these HL-miRNAs will provide a new venue for health and disease-related research in Drosophila.
|
| |
|
| Overall design |
Examination of mRNA levels in body tissues of young and old Drosophila melanogaster.
|
| |
|
| Contributor(s) |
Dhahbi JM |
| Citation(s) |
27042094 |
| Submission date |
Jan 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
joseph M dhahbi |
| E-mail(s) |
joseph.dhahbi@cnsu.edu
|
| Phone |
9166867891
|
| Organization name |
California Northstate University College of Medicine
|
| Department |
Medical Education
|
| Lab |
Biochemistry
|
| Street address |
9700 West Taron Drive
|
| City |
Elk Grove |
| State/province |
ca |
| ZIP/Postal code |
95757 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE77040 |
MicroRNAs circulate in the hemolymph of Drosophila and accumulate relative to tissue microRNAs in an age-dependent manner |
|
| Relations |
| BioProject |
PRJNA309308 |
| SRA |
SRP068676 |
Less...
More...