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| Status |
Public on Jan 21, 2019 |
| Title |
DNA methylation analysis of normal and HBx transgenic mouse liver |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
A genome-wide integrated DNA methylation analysis of the early stage of hepatocellular carcinoma development that induced by HBx. The HBV x (HBx) protein, which plays a critical role in the development of HCC, was shown to interact with several epigenetic factors, such as DNMT3A and HDAC1. Most HBx transgenic (TG) mice spontaneously develop HCC at about 1 year of age, providing genetic validation of the oncogenic potential of HBx even in the absence of viral integration and chronic inflammation. Therefore, it would be intriguing to study the regulatory role of HBx in the epigenome and its impact on HCC development. We performed a genome-wide analysis to examine the differences in DNA methylation enrichment pattern between cancer and normal liver cells.
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| |
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| Overall design |
Methylated-CpG island recovery assay (MIRA) of at 3 mouth old age mouse liver
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| |
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| Contributor(s) |
Lee S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 21, 2016 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Young-Joon Kim |
| E-mail(s) |
yjkim@yonsei.ac.kr
|
| Organization name |
Yonsei university
|
| Street address |
Yonsei-ro 50
|
| City |
Seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (2) |
| GSM2043195 |
DNA methylation analysis of normal mouse liver |
| GSM2043196 |
DNA methylation analysis of HBx transgenic mouse liver |
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| Relations |
| BioProject |
PRJNA309487 |
