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| Status |
Public on Sep 14, 2018 |
| Title |
The role of short telomeres in CD8 TEMRA cells (CCR7- CD45RA+) immune aging: A gene expression microarray study |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Telomere shortening is universally acquired with aging in humans, but the contribution of telomere shortening to immune aging is not fully understood. Here, we studied T cells derived from short telomere syndrome patients and compared their T cell profile and function to controls and elderly individuals who had normal age-adjusted telomere length. The short telomere syndrome patients were all under age 40 and carried mutant telomerase or telomere genes and had telomere lengths below the 1st age-adjusted percentile. The T cell profile of young short telomere syndrome patients resembled those that were five decades older including a depletion of naive T cell population and accumulation of terminally differentiated effector cells. To test whether short telomeres affect the quality of these cells, we performed a gene expression microarray. The data are summarized here. We found that although numerically expanded in both short telomere and elderly cases, the gene expression microarrays were distinct with short telomere cells predominately upregulating gene expression of DNA damage pathways and elderly T cells upregulating cell extrinsic apoptotic pathways.
The goal of the experiment is to highlight important genes and pathways that may drive immune aging in short telomere syndromes and which may also contribute to normal immune aging.
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| Overall design |
Human CD8+ TEMRA cells were flow sorted from short telomere syndrome subjects (test group), and two control groups, young (age-matched) and old subjects. Each test and control group contained 4 individual samples, 2 female and 2 male.
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| Contributor(s) |
Wagner CL, Talbot CC Jr, Armanios M |
| Citation(s) |
30179220 |
| Submission date |
Feb 03, 2016 |
| Last update date |
Sep 16, 2018 |
| Contact name |
Mary Armanios |
| E-mail(s) |
marmani1@jhmi.edu
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| Organization name |
The Johns Hopkins University
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| Department |
School of Medicine
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| Lab |
Medical Oncology
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| Street address |
401 North Broadway
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA310706 |
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