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| Status |
Public on Feb 03, 2017 |
| Title |
Regulation of matrix metalloproteinases (MMPs) expression and secretion by LIM and SH3 protein (LASP1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The process of tumor invasion requires degradation of extracellular matrix by proteolytic enzymes. Cancer cells form protrusive invadopodia, which produce and release matrix metalloproteinases (MMPs) to degrade the basement membrane and thereby allowing metastasis. We investigated the effect of LASP1, a newly identified protein in invadopodia, on expression, secretion and activation of MMPs in invasive breast tumor cells.
By analyzing and verifying microarray data sets and performing reporter assays, we demonstrate that LASP1 positively modulates the expression of c-Fos and AP-1 activity, and thereby up-regulates expression of MMP1, -3 and -9 in breast cancer cells. Furthermore, zymography assays and Western blot analysis revealed cytosolic LASP1 promotion of MMP vesicle secretion into the extracellular matrix, thus altering the microenvironment during cancer progression.
The newly identified role of LASP1 in regulating matrix degradation by affecting MMP transcription and secretion elucidated the migratory potential observed in studies that investigated the upregulation of LASP1 in aggressive cancers.
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| Overall design |
total samples analysed are 4
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| Contributor(s) |
Butt E, Kneitz S, Endres M, Perera RK |
| Citation(s) |
27588391 |
| Submission date |
Feb 04, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Susanne Elma Kneitz |
| E-mail(s) |
susanne.kneitz@uni-wuerzburg.de
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| Phone |
+49-931-31 86526
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| Organization name |
University of Wuerzburg
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| Department |
Physiologigcal Chemistry
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| Street address |
Biozentrum, Am Hubland
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| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA310869 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE77584_RAW.tar |
8.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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