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| Status |
Public on Feb 19, 2016 |
| Title |
Expression data from IMQ-induced psoriasis-like skin inflammation in miR-146a-/- and C57BL6J mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
miR-146a acts as a negative feedback regulator of inflammation. To investigate the role of miR-146a in psoriasis psoriasiform skin inflammation was indeuced in Mir-146a-/- and wild type mice (C57BL6J) by topical applciation of imiquimod (IMQ)-cream (Aldara).
Gene expression profiling (Affymetrix) was used to identify transcriptomic changes associated with psoriasis-like skin inflammation in wild type vs. miR-146a -/-mice.
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| Overall design |
A daily topical dose of 31.25 mg of Aldara cream (5% IMQ) was applied on the right ear of miR-146a -/- and C57BL/6 mice on three consecutive days to induce psorisis-like skin inflammation. Mice were sacrificed at day 4. Ear flaps were collected for total RNA extraction and hybridization on Affymatrix GeneTitan plate format Gene ST 2.1 (mouse).
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| Contributor(s) |
Srivastava A, Sonkoly E, Pivarcsi A |
| Citation(s) |
27568078 |
| Submission date |
Feb 18, 2016 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ankit Srivastava |
| E-mail(s) |
ankit.srivastava@ki.se
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| Phone |
+46767563207
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| Organization name |
Karolinska Institutet
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| Department |
Department of Medicine
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| Lab |
Molecular Dermatology Research Group
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| Street address |
CMM L8:02
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| City |
Stockholm |
| State/province |
- Select a State - |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA312479 |
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