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| Status |
Public on Dec 07, 2016 |
| Title |
Telomeric retrotransposon HeT-A contains a bidirectional promoter that initiates divergent transcription of piRNA precursors in Drosophila germline |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
PIWI-interacting (pi) RNAs provide silencing of transposable elements (TE) in the germline. Drosophila telomeres are maintained by transpositions of specialized telomeric retroelements. piRNAs generated from sense and antisense transcripts of telomeric elements provide telomere length control in the germline. Previously, we have found that antisense transcription of the major telomeric retroelement HeT-A is initiated upstream of the HeT-A sense transcription start site. Here, we performed a deletion analysis of the HeT-A promoter and show that common regulatory elements are shared by sense and antisense promoters of HeT-A. Therefore, the HeT-A promoter is a bidirectional promoter capable of processive sense and antisense transcription. Ovarian small RNA data shows that a solo HeT-A promoter within an euchromatic transgene initiates divergent transcription of transgenic reporter genes and subsequent processing of these transcripts into piRNAs. These events lead to the formation of a divergent uni-strand piRNA cluster at solo HeT-A promoters, in contrast to endogenous telomeres that represent strong dual-strand piRNA clusters. Solo HeT-A promoters are not immunoprecipitated with Heterochromatin Protein 1 (HP1) homolog Rhino, a marker of the dual-strand piRNA clusters, but are associated with HP1 itself, which provides piRNA-mediated transcriptional repression of the reporter genes. Unlike endogenous dual-strand piRNA clusters, the solo HeT-A promoter does not produce overlapping transcripts. In a telomeric context, however, bidirectional promoters of tandem HeT-A repeats provide read-through transcription of both genomic strands, followed by Rhi binding. These data indicate that Drosophila telomeres share properties of uni-strand and dual-strand piRNA clusters.
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| Overall design |
The fractions of small RNAs (19-29 nt) from ovaries of 3 transgenic strains of Drosophila melanogaster were sequenced using Illumina HiSeq 2000.
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| Contributor(s) |
Ryazansky S, Akulenko N, Kalmykova A |
| Citation(s) |
27939293 |
| Submission date |
Feb 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sergei Ryazansky |
| E-mail(s) |
s.ryazansky@gmail.com
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| Phone |
+7-499-196-81-73
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| Organization name |
Institute of Molecular Genetics
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| Department |
Department of Molecular Genetics of Cell
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| Street address |
Kurchatov sq., 2
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| City |
Moscow |
| ZIP/Postal code |
123182 |
| Country |
Russia |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (3) |
| GSM2067889 |
Small RNAs from ovaries of H90 transgenic strain |
| GSM2067890 |
Small RNAs from ovaries of H95 transgenic strain |
| GSM2067891 |
Small RNAs from ovaries of H111 transgenic strain |
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| Relations |
| BioProject |
PRJNA312711 |
| SRA |
SRP070611 |
