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| Status |
Public on Feb 23, 2016 |
| Title |
Expression data from LNCaP cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Prostate Cancer Stem Cells (CSCs) are considered one of the main reasons the tumor recurrence after chemotherapy. Here we employed a chemoresistant xenograft prostate cancer model in NOD/SCID mice and found CD54 is a reliable new marker for prostate CSCs. Gene expression profile were utilized to analyze stemness-related genes in cancer stem cells.
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| Overall design |
12 Male NOD/SCID mice were obtained from the Animal Center of the Chinese Academy of Medical Science (Beijing, China). For xenograft generation, 1×106 LNCaP cells were subcutaneously injected into the backs of NOD/SCID mice (n=24). When tumors reached a diameter of 5 mm after 3 d, mice were randomly divided into two groups without castration (12 per group). Mice in the treatment group were administered with cisplatin at a dose of 5 mg/kg every 3 d for 30 d. Mice in the control group were treated with vehicle PBS. Tumors were taken out for microarray assay after 30 d.
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| Contributor(s) |
Li C |
| Citation missing |
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| Submission date |
Feb 23, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Chong Li |
| E-mail(s) |
lichong87@126.com
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| Organization name |
Institute of Biophysics, Chinese Academy of Sciences
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| Street address |
15 Datun Road
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA312902 |
