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| Status |
Public on Dec 13, 2007 |
| Title |
Expression data from immature pDC and pDC activated with CpG 1826 and influenza virus PR8 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CpG 1826 binds to Toll-like receptor (TLR)9, whereas influenza virus PR8 activates pDC via TLR7. Differential stimulation of pDCs is expected to result in unique activation mechanism(s) leading to a different phenotypically and functionally matured pDC
We used microarrays to detail the global programme of gene expression underlying the maturation process of pDC activated with CpG 1826 and influenza virus PR8. We identified a distinct expression profile of upregulated immunomediators.
Keywords: time course
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| Overall design |
Sorted pDCs were cultured for 1h and 4hs in medium control or with 5 µg/ml CpG 1826 or 300 HAU/ml purified influenza A/PR/8 virus. The first experiment (e1) included pDC in media and stimulated with CpG for 4h. In two other independent experimental batches (e2 and e3), we obtained samples of sorted pDC cultured in medium alone (med), and with CpG or PR8 (flu) for 1h and 4h. RNA extraction was performed using the RNeasy Kit (Qiagen) and hybridization on Affymetrix microarrays was performed using standard protocols. We sought to obtain homogeneous populations of pDCs at different time points under defined activation conditions in order to decipher the temporal resolution of expression profiles during the process of their maturation.
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| Contributor(s) |
Weninger W, Iparraguirre A, Tobias JW |
| Citation(s) |
18029397 |
| Submission date |
May 17, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Amaya I Wolf |
| E-mail(s) |
aiwolf@wistar.org
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| Organization name |
Wistar Institute
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| Street address |
3601 Spruce street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (14)
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| GSM190134 |
pDC CpG 1h, biological rep2 |
| GSM190135 |
pDC flu 1h, biological rep1 |
| GSM190136 |
pDC flu 1h, biological rep2 |
| GSM190137 |
pDC medium 4h, biological rep1 |
| GSM190138 |
pDC medium 4h, biological rep2 |
| GSM190139 |
pDC medium 4h, biological rep3 |
| GSM190140 |
pDC CpG 4h, biological rep1 |
| GSM190141 |
pDC CpG 4h, biological rep2 |
| GSM190142 |
pDC CpG 4h, biological rep3 |
| GSM190143 |
pDC flu 4h, biological rep1 |
| GSM190144 |
pDC flu 4h, biological rep2 |
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| Relations |
| BioProject |
PRJNA100069 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7831_RAW.tar |
51.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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