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| Status |
Public on Jun 21, 2016 |
| Title |
Regulation of Th17 cell pathogenic function by miR-183/96/182 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Th17 cells are key players in autoimmune diseases. However, the roles of non-coding RNAs in Th17 cells are largely unknown. Here, we show that deletion of the Dicer gene specifically in Th17 cells protects from experimental autoimmune encephalomyelitis (EAE). Th17 cells highly express the miR-183/96/182 cluster (miR-183C), in response to IL-6/STAT3 signaling. Moreover, miR-183C regulates pathogenic cytokine expression during Th17 development. Furthermore, transcription factor Foxo1 is one of functional targets of miR-183C in Th17 cells: Foxo1 negatively regulates the pathogenicity of Th17 cells and miR-183C represses Foxo1 expression. Collectively, our results demonstrate one of crucial roles for miR-183C cluster in regulation of Th17 cell function in autoimmune diseases.
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| Overall design |
Control vector- or miR-96-overexpressing T cells and miR-183C+/+ or miR-183C-/- T cells were cultured under the Th17(23) condition and were harvested on day 4-5. Cell sorting was performed using a FACSAria cell sorter to obtain GFP-positive cells. Total RNA was extracted using the TRIzol reagent, enriched for polyA-containing RNAs and submitted to RNA-Seq.
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| Contributor(s) |
Kenji I, Alicia G, Chen D, Changchun X |
| Citation(s) |
27332731 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI087634 |
The Role of miR-17~92 in Autoimmune Diseases |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| R01 AI089854 |
Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| R01 AI121155 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| R56 AI110403 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
| R56 AI121155 |
Regulation of T Follicular Helper Cell Differentiation by MicroRNAs |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
|
| Submission date |
Feb 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Changchun Xiao |
| E-mail(s) |
cxiao@scripps.edu
|
| Phone |
(858) 784-7640
|
| Organization name |
The Scripps Research Institute
|
| Department |
Immunology & Microbial Science
|
| Lab |
Changchun Xiao Lab
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA313159 |
| SRA |
SRP070814 |
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