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Status |
Public on Mar 10, 2017 |
Title |
Cell density effects on normal astrocytes and glioma cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Glioma cells and normal astrocytes were plated at low and high density to compare density-dependent gene expression.
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Overall design |
Primary glioma cells were splated at either sparse plating density (5x104 cells/mL) or dense (3x105 cell/mL). NHAs were plated at either sparse plating density (3.5x105 cells/55cm2 dish) or dense (5.2x106/55cm2 dish) at a final conc of 5x104 cells/mL and 3x105 cell/mL. Cells were incubated for 5 days prior to RNA harvest.
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Contributor(s) |
Stommel JM |
Citation(s) |
28118603, 31664073 |
Submission date |
Mar 11, 2016 |
Last update date |
Nov 12, 2019 |
Contact name |
Jayne M Stommel |
E-mail(s) |
jayne.stommel@nih.gov
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Organization name |
National Cancer Institute
|
Street address |
10 Center Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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Samples (28)
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Relations |
BioProject |
PRJNA314942 |