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| Status |
Public on Jun 22, 2009 |
| Title |
In vitro modeling of primordial germ cell development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transgenic StellaGFP ESCs were used to derive primordial germ cells during embryoid body (EB) differentiation, and microarry analysis used to compared FACS sorted Stella-positive cells of day 7 Ebs with the parental ESCs and Stella-negative cells of day 7 Ebs.
Keywords: in vitro, primordial germ cells, embryonic stem cells, stella
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| |
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| Overall design |
Four replicates of Stella-positive day 7 cells were compared to two replicates of stella-negative day 7 cells. Also, there were three replicated of FACS sorted Stella-negative ESCs, two replicated of FACS-sorted Stella-postive ESCs, and two replicates of unsorted StellaGFP ESCs.
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| |
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| Citation(s) |
19578360 |
| Submission date |
May 29, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jason Allen West |
| E-mail(s) |
jasonallenwest@gmail.com
|
| Organization name |
Children's Hospital, Boston
|
| Street address |
One Blackfan Circle Room 7004B
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (13)
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|
| Relations |
| BioProject |
PRJNA100127 |
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