|
| Status |
Public on Mar 08, 2017 |
| Title |
Single cell sequencing of Dgcr8 knockout embryonic stem cells plus/minus miR-294 or let-7 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed Fluidigm C1 single cell sequencing analysis of wild-type and microRNA deficient (Dgcr8 knockout) mouse embryonic stem cells mock treated or transfected with either miR-294 or let-7.
|
| |
|
| Overall design |
Wild-type and Dgcr8 knockout cells grown in naïve culture conditions were mock transfected or transfected with miRNA mimics for let-7b or miR-294, single cells were captured on Fluidigm C1 24 hours post-transfection and then prepared for sequencing on Illumina HiSeq1000 following manufacturer's protocol.
|
| |
|
| Contributor(s) |
Blelloch R, dii Bernardo D |
| Citation(s) |
28102192 |
| Submission date |
Apr 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Annamaria Carissimo |
| E-mail(s) |
carissimo@tigem.it
|
| Organization name |
Tigem
|
| Street address |
via Castellino
|
| City |
Napoli |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
|
| Samples (232)
|
|
| Relations |
| BioProject |
PRJNA318175 |
| SRA |
SRP073181 |
Less...
More...