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| Status |
Public on Apr 28, 2018 |
| Title |
Evolved Repression Overcomes Enhancer Robustness |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Biological systems display extraordinary robustness. Robustness of transcriptional enhancers results mainly from clusters of binding sites for the same transcription factor, and it is not clear how robust enhancers can evolve loss of expression through point mutations. Here, we report the high-resolution functional dissection of a robust enhancer of the shavenbaby gene that has contributed to morphological evolution. We found that robustness is encoded by many binding sites for the transcriptional activator Arrowhead and that, during evolution, some of these activator sites were lost, weakening enhancer activity. Complete silencing of enhancer function, however, required evolution of a binding site for the spatially restricted potent repressor Abrupt. These findings illustrate that recruitment of repressor binding sites can overcome enhancer robustness and may minimize pleiotropic consequences of enhancer evolution. Recruitment of repression may be a general mode of evolution to break robust regulatory linkages.
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| Overall design |
8 samples are analyzed: background GFP- and target GFP+ cells from four independent sortings.
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| Contributor(s) |
Preger-Ben Noon E, Davis FP, Stern DL |
| Citation(s) |
27840106 |
| Submission date |
Apr 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ella Preger-Ben Noon |
| E-mail(s) |
pregere@janelia.hhmi.org
|
| Phone |
5712093250
|
| Organization name |
HHMI Janelia Research Campus
|
| Lab |
David Stern
|
| Street address |
19700 Helix Drive
|
| City |
Ashburn |
| State/province |
VA |
| ZIP/Postal code |
20147 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA319982 |
| SRA |
SRP074148 |
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