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| Status |
Public on Nov 16, 2016 |
| Title |
Expression data for analysis of genes regulated by EWS/FLI1 protein levels in Ewing sarcoma cell line A673 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Comparison of gene expression profile of Ewing sarcoma cells which have an exchange of the endogenous EWS/FLI1 to either wild-type or a turnover-deficient mutant EWS/FLI1. Most target genes are saturated as only a few target genes are soly driven by increasing protein amount.
The effect of a stable EWS/FLI1 mutant on global gene expression was evaluated in A673 Ewing sarcoma cells.
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| Overall design |
A doxycycline-inducible shRNA construct directed against the 5'UTR of endogenous EWS/FLI1 was stably transduced into A673 cells and used for silencing. Further, a doxycycline-inducible overexpression construct was used to simultaneously express either a flag-tag, 3xflag-EWS/FLI1 or 3xflag-EWS/FLI1 K380R. RNA used for expression profiling was isolated from these A673 cells after 24h and 48h doxycycline-induction. Controls cells were uninduced samples from each cell line construct.
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| Contributor(s) |
Schäfer BW, Gierisch ME, Wachtel M |
| Citation(s) |
27875302 |
| Submission date |
May 02, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Maria Elka Gierisch |
| E-mail(s) |
maria.gierisch@kispi.uzh.ch
|
| Organization name |
University Children's Hospital Zurich
|
| Department |
Department of Oncology
|
| Street address |
Steinwiesstrasse 32
|
| City |
Zurich |
| ZIP/Postal code |
8032 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
|
| GSM2140720 |
control cells (1), time point 48h, uninduced |
| GSM2140721 |
shRNA directed against EWS/FLI1, expression of 3xflag ctr 24h |
| GSM2140722 |
shRNA directed against EWS/FLI1, expression of 3xflag ctr 48h |
| GSM2140723 |
control cells (2), time point 48h, uninduced |
| GSM2140724 |
shRNA directed against EWS/FLI1, expression of 3xflag-EWS/FLI1 24h |
| GSM2140725 |
shRNA directed against EWS/FLI1, expression of 3xflag-EWS/FLI1 48h |
| GSM2140726 |
control cells (3), time point 48h, uninduced |
| GSM2140727 |
shRNA directed against EWS/FLI1, expression of 3xflag-EWS/FLI1 K380R 24h |
| GSM2140728 |
shRNA directed against EWS/FLI1, expression of 3xflag-EWS/FLI1 K380R 48h |
|
| Relations |
| BioProject |
PRJNA320295 |
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