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| Status |
Public on Feb 24, 2017 |
| Title |
Expression data from human T cell populations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Murine T cell populations have been described that are generated upon strong TCR activation, a process referred to as agonist selection. This murine population is phenotypically and functionally characterized and lacks coreceptors CD4 and CD8 (double negative or DN). The existence of a human equivalent of this population is not clear.
We identified a population based on expression of PD-1, CD10 and lower expression of CD3 that shows characteristics resemblant of mouse DN T cells. Here, we perform transcriptional profiling of this particular PD-1+ T cell population in human thymus and cord blood and compare it to conventional CD8 T cells (that are PD-1 negative) as well as innate TCRgammadelta+ T cells.
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| Overall design |
We purified PD-1- CD8+and PD-1+CD8+ populations from human donor post-natal thymus and cord blood as well as TCRgd+T cells from human post-natal thymus. Purification was based on ex vivo fluorescence-activated cell sorting (FACS) of mononuclear cell fractions obtained from donor samples. For each population 3 donors were used and obtained fractions were pooled for further analysis.
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| Contributor(s) |
Verstichel G, Vandekerckhove B |
| Citation(s) |
28783686 |
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The checkpoint for agonist selection precedes conventional selection in human thymus. Greet Verstichel, David Vermijlen, Liesbet Martens, Glenn Goetgeluk, Margreet Brouwer, Nicolas Thiault, Yasmine Van Caeneghem, Stijn De Munter, Karin Weening, Sarah Bonte, Georges Leclercq, Tom Taghon, Tessa Kerre, Yvan Saeys, Jo Van Dorpe, Hilde Cheroutre and Bart Vandekerckhove. Science Immunology 24 Feb 2017. Vol. 2, Issue 8, eaah4232. DOI: 10.1126/sciimmunol.aah4232
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| Submission date |
May 04, 2016 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Bart Vandekerckhove |
| E-mail(s) |
bart.vandekerckhove@ugent.be
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| Organization name |
Ghent University
|
| Department |
Department of Clinical chemistry, Microbiology and immunology
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| Lab |
Prof. dr. Vandekerckhove
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| Street address |
MRB2 1e verdieping, De Pintelaan 185
|
| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA320524 |
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