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| Status |
Public on Aug 24, 2017 |
| Title |
DNase Hypersensitivity Peaks from Rat Schwann Cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We utilized DNase-Seq to identify open chromatin regions within Schwann cells. This data was used in conjunction with sequence conservation and transcription factor binding site prediction to identify putative cis-regulatory elements critical for Schwann cells. We used three independent biological replicates, and for our studies used sample 'barcode4' as a representative sample for all three. DNase HSS peaks were identified using F-Seq and HOMER, and overlapped to prioritize candidate regions. These regions were then verified using luciferase assays.
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| |
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| Overall design |
Performed DNase HSS on three independent biological replicates of S16 rat Schwann cells
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| |
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| Contributor(s) |
Law WD, Antonellis A |
| Citation(s) |
27821050 |
| Submission date |
May 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Anthony Antonellis |
| E-mail(s) |
antonell@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Human Genetics
|
| Street address |
1241 E Catherine St.
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA322264 |
| SRA |
SRP075452 |
