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| Status |
Public on Dec 22, 2017 |
| Title |
Temporal Transcriptomic and Proteomic Landscapes of Deteriorating Pancreatic Islets in Type 2 Diabetic Rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Progressive reductions in β-cell mass and function comprise the core of the pathogenesis mechanism leading to type 2 diabetes (T2D). To understand the molecular events in this process, we quantified the temporal transcriptome and proteome of pancreatic islets from Goto-Kakizaki (GK) rats at different stages of diabetes. Integrated omics analysis allowed us to unravel the chronological order of T2D-related molecular events during GK islet deterioration. Two major events occur early in the disease, specifically, a reduction in β-cell mass caused by defective neogenesis and senescence-related low proliferation, and metabolic shift caused by mitochondrial dysfunction. Furthermore, our data revealed the evolution of compensation failure in GK islets and two distinct stages of islet inflammation: priming and amplification. Our study offers a valuable resource for the diabetes research community and will facilitate further studies aimed at protecting β-cell mass and function.
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| Overall design |
We performed a time-course large-scale transcriptome of pancreatic islets in GK male rats vs control, age-matched WST male rats at five time points (week 4, 6, 8, 16 and 24) using MAPS method
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| Contributor(s) |
Hou J, Li Z, Zhong W, Hao Q, Zhao D, Xu P, Zhou Y, Wang Y, Xu T |
| Citation(s) |
28559245 |
| Submission date |
May 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Wen Zhong |
| E-mail(s) |
zhongwenhust@gmail.com
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| Organization name |
Institute of Biophysics, Chinese Academy of Sciences
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| Street address |
Datun Road
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA322601 |
| SRA |
SRP075609 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE81811_RAW.tar |
596.5 Mb |
(http)(custom) |
TAR (of BIGWIG, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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