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| Status |
Public on May 24, 2017 |
| Title |
Gene expression signatures for TCM Suduxing treated HepG2.2.15 cells compared to Suduxing untreated HepG2.2.15 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To further analyze the gene expression profile regulated by TCM Suduxing, we employed whole genome microarray expression profiling as a discovery platform to identify the differentially expressed genes. Human hepatocellular carcinoma cell lines HepG2.2.15 cells were treated for 48 hr with 0.001 μg/ml and 0.01μg/ml Suduxing or without Suduxing(control samples) in vitro. The differentially expressed genes including all detected and normalized signal more than 8 were selected and 538 upregulated- and downregulated- genes were identified between 0.01μg/ml dose and control samples.Afterwards, the differentially-expressed genes were analyzed using KEGG database (http://www.genome.jp/kegg/) and 10 genes related to HBV infection-related molecular interaction network were identified.
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| Overall design |
Suduxing-regulated genes in HepG2.2.15 cells was measured at 48 hours after exposure to doses of 0 μg/ml, 0.001 μg/ml,and 0.01 μg/ml.Three independent experiments were performed at each time (48 hours) using different doses for each experiment.
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| Contributor(s) |
Liu Y, Yao W, Xu D |
| Citation missing |
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| Submission date |
May 24, 2016 |
| Last update date |
May 25, 2017 |
| Contact name |
dongping xu |
| E-mail(s) |
xudongping302@sina.com
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| Organization name |
Research Center for Clinical and Translational Medicine, Beijing 302 Hospital, Beijing
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| Street address |
#100 Xisihuan Middle Road, Beijing
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| City |
Beijing |
| ZIP/Postal code |
100039 |
| Country |
China |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA322672 |
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