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| Status |
Public on May 01, 2019 |
| Title |
Dopamine receptor allows selective targeting of neoplastic progenitors in human acute myeloid leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Dopamine receptor (DRD) antagonist thioridazine (TDZ) has been traditionally prescribed as an anti-psychotic drug. Recent observations have revealed anti-neoplastic effects of TDZ in a variety of neural and non-neural cancers including acute myeloid leukemia (AML). However, the basis of TDZ effects on transformed tissues is not fully understood. We used AML as a model system to study the anti-neoplastic properties of TDZ, as well as the downstream mechanism of action for DRDs in the context of cancer. Our study defines a role for DRDs in regulating neoplastic properties and suggests DRD2 as an attractive therapeutic target for AML.
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| Overall design |
Xenografted AML samples were obtained after in vivo treatment with Thioridazine (TDZ) for 21 days. The human leukemic cells (hCD45+CD33+) were FACS-purified and RNA was extracted. Gene expression profiles were compared between TDZ- versus vehicle (captisol 30%)-treated matched xenografts.
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| Contributor(s) |
Aslostovar L, Benoit YD, Boyd AL, Shapovalova Z, Reid JC, Bhatia M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 31, 2016 |
| Last update date |
May 22, 2019 |
| Contact name |
Mickie Bhatia |
| E-mail(s) |
mbhatia@mcmaster.ca
|
| Organization name |
McMaster University
|
| Department |
Stem Cell and Cancer Research Institute (SCC-RI)
|
| Street address |
1200 Main Street West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8N 3Z5 |
| Country |
Canada |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| GSM2182726 |
AML#1 leukemic xenograft_Control_Recipient mouse 2 |
| GSM2182727 |
AML#1 leukemic xenograft_Control_Recipient mouse 1 |
| GSM2182728 |
AML#1 leukemic xenograft_TDZ |
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| Relations |
| BioProject |
PRJNA323841 |
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