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| Status |
Public on Jun 02, 2016 |
| Title |
Bone marrow-derived macrophage responses to classical activation: wild-type vs. MLL1-deficient (Lys2Cre MLL1 fx/fx) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
mRNA from wild-type (Cre-) and MLL1-deficient (Cre+) BMDMs were analyzed via gene chip (Mouse Gene ST 2.1, Affymetrix) for relative expression changes. Isolated mRNA from Cre- and Cre+ BMDMs stimulated with classical activation signals (IFNg, LPS or IFNg+LPS) was analyzed using a gene chip panel of >40,000 RefSeq transcripts, and resulting fold expression was determined by analyzing quality-controlled expression values for validated probesets.
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| Overall design |
Bone marrow derived macrophages from wild-type (Cre-) or MLL1-deficient (Cre+) mice were stimulated in vitro with IFNgamma (10 ng/ml), LPS (100 ng/ml) or the combination of IFNgamma+LPS for six hours. Cells were then processed in Trizol reagent for RNA extraction.
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| Contributor(s) |
Carson IV WF |
| Citation(s) |
28733487 |
| Submission date |
Jun 01, 2016 |
| Last update date |
May 29, 2018 |
| Contact name |
William Franklin Carson |
| E-mail(s) |
wfcarson@umich.edu
|
| Phone |
7349361020
|
| Organization name |
University of Michigan Medical School
|
| Department |
Pathology
|
| Street address |
109 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (24)
|
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| Relations |
| BioProject |
PRJNA324066 |
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