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| Status |
Public on Jun 01, 2017 |
| Title |
High-Fat High-Sugar Diet Induces Polycystic Ovary Syndrome in a Rodent Model |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Obesity has been linked with a host of metabolic and reproductive disorders including polycystic ovary syndrome (PCOS). While a distinct link exists between obesity and PCOS, the exact pathogenesis of the disease remains less understood and limited research has explored the impact of diet on the development of PCOS. With the primary symptoms of PCOS including hyperandrogenism, anovulation, and polycystic ovaries, most animal models utilize androgen treatment to effectively induce PCOS. However, these models fail to address the underlying causes of disease symptoms and do not effectively demonstrate the metabolic features of the disease such as hyperinsulinemia. Here, we present a novel rodent model of diet-induced obesity that recapitulates both the metabolic and reproductive phenotypes of human PCOS. In utilizing a high-fat high-sugar (HFHS) diet, we have created a model of PCOS that allows for the study of metabolic parameters and their impact on ovarian follicle development and reproductive health. Animals on the HFHS diet not only demonstrated signs of metabolic impairment, but they also developed polycystic ovaries and experienced irregular estrous cycling marked by an extended period spent in estrus. Though hyperandrogenism was not characteristic of HFHS diet animals as a group, testosterone levels were predictive of a polycystic ovarian morphology. Importantly, PCOS was induced similarly to the disease etiology in humans, allowing this model to offer the unique opportunity to study PCOS at its genesis rather than following the development of disease symptoms.
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| Overall design |
Female Sprague-Dawley rats (n = 60; P17) were randomly assigned to either a HFHS (n = 32) or control diet (n = 28) group. The control group had ad libitum access to water and standard rat chow (Envigo – LM-485, 3.1 kcal/g, 17% calories from fat). Rats were bilaterally ovariectomized after 11 weeks of diet exposure. For RNA-Seq analysis, two ovaries were collected from two different animals in each experimental group.
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| Contributor(s) |
Roberts JS, Bowman JJ, Ozark PA, Blythe SN, Toporikova N, Whitworth GB |
| Citation(s) |
28203719 |
| Submission date |
Jun 10, 2016 |
| Last update date |
May 10, 2023 |
| Contact name |
Gregg Brooks Whitworth |
| E-mail(s) |
whitworthg@wlu.edu
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| Phone |
(415) 336-3872
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| Organization name |
Washington and Lee Unversity
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| Department |
Biology
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| Street address |
204 West Washington St
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| City |
Lexington |
| State/province |
VA |
| ZIP/Postal code |
24450 |
| Country |
USA |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA325290 |
| SRA |
SRP076384 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83220_RAW.tar |
1.7 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE83220_fpkm-ovaries.csv.gz |
234.8 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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