|
| Status |
Public on Jun 15, 2016 |
| Title |
Expression data from ELK3 knocked down MDA-MB-231 cell line. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Triple-negative breast cancer is a highly aggressive tumor subtype that lacks effective therapeutic targets. Here, we show that ELK3 is overexpressed in a subset of breast cancers, in particular basal-like and normal-like/claudin-low cell lines. Suppression of ELK3 in MDA-MB-231 cells led to transdifferentiation from an invasive mesenchymal phenotype to a non-invasive epithelial phenotype both in vitro and in vivo.
Suppression of ELK3 results in the extensive changes in genome expression profiles. Among these, GATA3, a master suppressor of metastasis, was epigenetically activated and we found that suppression of GATA3 led to the restoration of migration and invasion. These results suggest that the ELK3-GATA3 axis is a major pathway that promotes metastasis of MDA-MB-231 cells.
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| Overall design |
Retrovirus expressing shRNA of ELK3 was transduced into MDA-MB-231 cell line and stable cell line of which ELK3 is suppressed more than 50% was selected by the drug selection (Puromycin).
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| Contributor(s) |
Park K, Kim K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 14, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Jae Yong Lee |
| Organization name |
Cha Unniversity
|
| Street address |
335, Pangyo-ro
|
| City |
SungNam |
| ZIP/Postal code |
13488 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
|
| Samples (4)
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| GSM2199543 |
Stable MDA-MB-231 cell line established by the integration of control shRNA, Biological repeat 1 |
| GSM2199546 |
Stable MDA-MB-231 cell line established by the integration of control shRNA, Biological repeat 2 |
| GSM2199547 |
Stable MDA-MB-231 cell line established by the integration of shRNA targeting to ELK3, Biological repeat 1 |
| GSM2199548 |
Stable MDA-MB-231 cell line established by the integration of shRNA targeting to ELK3, Biological repeat 2 |
|
| Relations |
| BioProject |
PRJNA325597 |
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