|
| Status |
Public on Aug 07, 2016 |
| Title |
Modeling human brain evolution using induced pluripotent stem cells: comparative analysis of neuronal development in humans and chimpanzees |
| Organisms |
Pan troglodytes; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding evolutionary mechanisms underlying expansion and reorganization of the human brain represents an important aspect in analyzing the emergence of cognitive abilities typical of our species. Comparative analyses of neuronal phenotypes in closest living relatives (Pan troglodytes; the common chimpanzee) can shed the light into changes in neuronal morphology compared to the last common ancestor (LCA), opening possibilities for analyses of the timing of their appearance, and the role of evolutionary mechanisms favoring a particular type of information processing in humans. Here, we use induced pluripotent stem cell (iPSC) technology to model neural progenitor cell migration and early development of cortical pyramidal neurons in humans and chimpanzees. In addition, we provide morphological characterization of the early stages of neuronal development in human and chimpanzee transplanted cells, and examine the role of developmental mechanisms previously proposed for the evolutionary expansions of the human brain on the early development of pyramidal neurons in the two species. The strategy proposed here lay down the basis for further comparative analysis between human and non-human primates and opens new avenues for understanding cognitive capability and neurological disease susceptibility differences between species.
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| |
|
| Overall design |
PolyA RNA-Seq profiling of neural progenitor cells (NPCs) and neurons differentiated from human and chimpanzee iPSCs.
|
| |
|
| Contributor(s) |
Narvaiza I, Marchetto MC, Benner C, Gage FH |
| Citation(s) |
27487209 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 MH095741 |
Single-Cell Approaches to Reveal How Jumping Genes Individualize Neural Circuits |
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES |
GAGE |
|
| Submission date |
Jun 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Inigo Narvaiza |
| E-mail(s) |
narvaiza@salk.edu
|
| Organization name |
Salk Institute
|
| Department |
Laboratory of Genetics
|
| Lab |
Gage
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (4)
|
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL16809 |
Illumina HiSeq 2000 (Pan troglodytes) |
| GPL19148 |
Illumina HiSeq 2500 (Pan troglodytes) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA326545 |
| SRA |
SRP076951 |
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